BIOD 171MICRO LAB EXAMS

1. What three elements are used in an autoclave to sterilize equipment?
2. What is the minimum temperature an autoclave must be set at to achieve sterile condition?
3. If you are working in a lab in which an autoclave is not available, and you are pressed for time,
which would you chose to best sterilize your equipment? Hot steam or hot air? Explain why you
chose your answer.
4. What type of incubator is pictured below? Fixed incubator
5. At what temperature is the fixed incubator set to, as presented in the lab video?
6. At what temperature should you refrigerate bacterial samples? Explain why this is beneficial.
7. What are the FOUR types of gloves presented in the lab video
8. What THREE rules were discussed in regards to lab safety that would protect you and others from
contamination?

9. What are the main sections that should be found in a lab notebook? Name at least 4. Objective,

LAB 2
4. How should you hold the microscope when you move it or carry it?
5. List the four magnification strengths found on the objectives of the light microscope shown in the
lab video.
6. As you looking through the microscope you notice your sample is slightly off-center—what
component of the microscope would be used to bring the sample into the center of the image?

7. What objective power is best suited if you are uncertain what the sample is and where to begin?

8. You are viewing a sample of bacteria that is 3 mm in diameter through a 40x objective lens. The
eyepiece has a magnification power of 10x. What size will the sample appear through the eyepiece?

9. Based on the microscope shown in the lab lecture, which objectives would NOT require placing oil
on the slide?
LAB 3 EXAM
1. List the 4 main steps used to prepare a WET mount and indicate which (if any) step is optional.